RPR Manual Procedure and Interpretation

Step 1

Using a fresh pipet, add one drop of serum or plasma onto a circle on the test card. Repeat by adding a drop of REACTIVE, WEAK REACTIVE, or NONREACTIVE CONTROL from the dropper vials.

Step 2

Using the flat end of the stirrer pipet, spread the sample over the entire area of the test circle.

Step 3

Attach the needle to the dropping bottle. Mix the Carbon Antigen. Dispense several drops into the dropping bottle cap to ensure a clear needle passage.

Step 4

Dispense one drop of the antigen suspension onto each sample. DO NOT RESTIR the sample and the antigen. Aspirate any antigen from the bottle cap.

Step 5

Place the card on an automatic rotator and cover for 8 minutes. When complete, physically rotate/tilt the test card 3-4 times until particles are fully dispersed.

Step 6

Immediately read results macroscopically in the “wet” state under a high intensity light source.